Production of the main surface antigen of Toxoplasma gondii in tobacco leaves and analysis of its antigenicity and immunogenicity.
Identifieur interne : 000785 ( Main/Exploration ); précédent : 000784; suivant : 000786Production of the main surface antigen of Toxoplasma gondii in tobacco leaves and analysis of its antigenicity and immunogenicity.
Auteurs : Marina Clemente [Argentine] ; Roberto Curilovic ; Alina Sassone ; Alicia Zelada ; Sergio O. Angel ; Alejandro N. MentaberrySource :
- Molecular biotechnology [ 1073-6085 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Anticorps antiprotozoaires (immunologie), Antigènes de protozoaire (administration et posologie), Antigènes de protozoaire (biosynthèse), Antigènes de protozoaire (génétique), Antigènes de protozoaire (immunologie), Encéphale (anatomopathologie), Encéphale (immunologie), Encéphale (parasitologie), Expression des gènes (MeSH), Femelle (MeSH), Feuilles de plante (génétique), Feuilles de plante (immunologie), Feuilles de plante (métabolisme), Immunoglobuline G (immunologie), Kystes (anatomopathologie), Kystes (immunologie), Kystes (parasitologie), Potexvirus (génétique), Protéines de protozoaire (administration et posologie), Protéines de protozoaire (biosynthèse), Protéines de protozoaire (génétique), Protéines de protozoaire (immunologie), Rhizobium (génétique), Souris (MeSH), Souris de lignée C3H (MeSH), Tabac (génétique), Tabac (immunologie), Tabac (métabolisme), Toxoplasma (génétique), Toxoplasma (immunologie), Toxoplasmose (immunologie), Toxoplasmose (prévention et contrôle).
- MESH :
- administration et posologie : Antigènes de protozoaire, Protéines de protozoaire.
- anatomopathologie : Encéphale, Kystes.
- biosynthèse : Antigènes de protozoaire, Protéines de protozoaire.
- génétique : Antigènes de protozoaire, Feuilles de plante, Potexvirus, Protéines de protozoaire, Rhizobium, Tabac, Toxoplasma.
- immunologie : Anticorps antiprotozoaires, Antigènes de protozoaire, Encéphale, Feuilles de plante, Immunoglobuline G, Kystes, Protéines de protozoaire, Tabac, Toxoplasma, Toxoplasmose.
- métabolisme : Feuilles de plante, Tabac.
- parasitologie : Encéphale, Kystes.
- prévention et contrôle : Toxoplasmose.
- Animaux, Expression des gènes, Femelle, Souris, Souris de lignée C3H.
English descriptors
- KwdEn :
- Animals (MeSH), Antibodies, Protozoan (immunology), Antigens, Protozoan (administration & dosage), Antigens, Protozoan (biosynthesis), Antigens, Protozoan (genetics), Antigens, Protozoan (immunology), Brain (immunology), Brain (parasitology), Brain (pathology), Cysts (immunology), Cysts (parasitology), Cysts (pathology), Female (MeSH), Gene Expression (MeSH), Immunoglobulin G (immunology), Mice (MeSH), Mice, Inbred C3H (MeSH), Plant Leaves (genetics), Plant Leaves (immunology), Plant Leaves (metabolism), Potexvirus (genetics), Protozoan Proteins (administration & dosage), Protozoan Proteins (biosynthesis), Protozoan Proteins (genetics), Protozoan Proteins (immunology), Rhizobium (genetics), Tobacco (genetics), Tobacco (immunology), Tobacco (metabolism), Toxoplasma (genetics), Toxoplasma (immunology), Toxoplasmosis (immunology), Toxoplasmosis (prevention & control).
- MESH :
- chemical , administration & dosage : Antigens, Protozoan, Protozoan Proteins.
- chemical , biosynthesis : Antigens, Protozoan, Protozoan Proteins.
- chemical , genetics : Antigens, Protozoan, Protozoan Proteins.
- chemical , immunology : Antibodies, Protozoan, Antigens, Protozoan, Immunoglobulin G, Protozoan Proteins.
- genetics : Plant Leaves, Potexvirus, Rhizobium, Tobacco, Toxoplasma.
- immunology : Brain, Cysts, Plant Leaves, Tobacco, Toxoplasma, Toxoplasmosis.
- metabolism : Plant Leaves, Tobacco.
- parasitology : Brain, Cysts.
- pathology : Brain, Cysts.
- prevention & control : Toxoplasmosis.
- Animals, Female, Gene Expression, Mice, Mice, Inbred C3H.
Abstract
We adapted a previously described Agrobacterium-mediated transient expression system to test the expression level of three constructs carrying the surface antigen 1 (SAG1) of Toxoplasma gondii. Two constructs were based in a Potato virus X (PVX) amplicon. In one of them, the PVX movement protein genes were replaced by the sag1 gene. In the other, the sag1 gene was placed under the control of an additional coat protein subgenomic promoter. In the third construct, the sag1 gene was fused to an apoplastic peptide signal under the CaMV 35S promoter. Western blot analysis of leaf extracts infiltrated with each construct revealed a protein of 35 kDa. SAG1 accumulation in leaves ranged from 0.1 to 0.06% of total soluble protein (equivalent to 10 microg and 6 microg of SAG1 per gram of fresh leaf tissue, respectively). Three of five human seropositive samples reacted with tobacco-expressed SAG1 in Western blot analysis. The C3H mice were immunized with SAG-expressing leaf extracts and perorally challenged with a nonlethal dose of the T. gondii Me49 strain. Mice vaccinated with SAG1 showed significantly lower brain cyst burdens compared to those from the control group. Immunization with SAG1-expressing leaves elicited a specific humoral response with predominant participation of type IgG2a. In conclusion, a functional SAG1 version could be transiently expressed in tobacco leaves.
DOI: 10.1385/MB:30:1:041
PubMed: 15805575
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Buenos Aires</wicri:noRegion></affiliation></author><author><name sortKey="Curilovic, Roberto" sort="Curilovic, Roberto" uniqKey="Curilovic R" first="Roberto" last="Curilovic">Roberto Curilovic</name></author><author><name sortKey="Sassone, Alina" sort="Sassone, Alina" uniqKey="Sassone A" first="Alina" last="Sassone">Alina Sassone</name></author><author><name sortKey="Zelada, Alicia" sort="Zelada, Alicia" uniqKey="Zelada A" first="Alicia" last="Zelada">Alicia Zelada</name></author><author><name sortKey="Angel, Sergio O" sort="Angel, Sergio O" uniqKey="Angel S" first="Sergio O" last="Angel">Sergio O. Angel</name></author><author><name sortKey="Mentaberry, Alejandro N" sort="Mentaberry, Alejandro N" uniqKey="Mentaberry A" first="Alejandro N" last="Mentaberry">Alejandro N. Mentaberry</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15805575</idno><idno type="pmid">15805575</idno><idno type="doi">10.1385/MB:30:1:041</idno><idno type="wicri:Area/Main/Corpus">000796</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000796</idno><idno type="wicri:Area/Main/Curation">000796</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000796</idno><idno type="wicri:Area/Main/Exploration">000796</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Production of the main surface antigen of Toxoplasma gondii in tobacco leaves and analysis of its antigenicity and immunogenicity.</title><author><name sortKey="Clemente, Marina" sort="Clemente, Marina" uniqKey="Clemente M" first="Marina" last="Clemente">Marina Clemente</name><affiliation wicri:level="1"><nlm:affiliation>IIB-INTECH, Camino de Circunvalación Laguna Km. 6, B7130IIWA, Chascomús, Prov. Buenos Aires, Argentina. mclemente@intech.gov.ar</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>IIB-INTECH, Camino de Circunvalación Laguna Km. 6, B7130IIWA, Chascomús, Prov. Buenos Aires</wicri:regionArea><wicri:noRegion>Prov. Buenos Aires</wicri:noRegion></affiliation></author><author><name sortKey="Curilovic, Roberto" sort="Curilovic, Roberto" uniqKey="Curilovic R" first="Roberto" last="Curilovic">Roberto Curilovic</name></author><author><name sortKey="Sassone, Alina" sort="Sassone, Alina" uniqKey="Sassone A" first="Alina" last="Sassone">Alina Sassone</name></author><author><name sortKey="Zelada, Alicia" sort="Zelada, Alicia" uniqKey="Zelada A" first="Alicia" last="Zelada">Alicia Zelada</name></author><author><name sortKey="Angel, Sergio O" sort="Angel, Sergio O" uniqKey="Angel S" first="Sergio O" last="Angel">Sergio O. Angel</name></author><author><name sortKey="Mentaberry, Alejandro N" sort="Mentaberry, Alejandro N" uniqKey="Mentaberry A" first="Alejandro N" last="Mentaberry">Alejandro N. Mentaberry</name></author></analytic><series><title level="j">Molecular biotechnology</title><idno type="ISSN">1073-6085</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Antibodies, Protozoan (immunology)</term><term>Antigens, Protozoan (administration & dosage)</term><term>Antigens, Protozoan (biosynthesis)</term><term>Antigens, Protozoan (genetics)</term><term>Antigens, Protozoan (immunology)</term><term>Brain (immunology)</term><term>Brain (parasitology)</term><term>Brain (pathology)</term><term>Cysts (immunology)</term><term>Cysts (parasitology)</term><term>Cysts (pathology)</term><term>Female (MeSH)</term><term>Gene Expression (MeSH)</term><term>Immunoglobulin G (immunology)</term><term>Mice (MeSH)</term><term>Mice, Inbred C3H (MeSH)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (immunology)</term><term>Plant Leaves (metabolism)</term><term>Potexvirus (genetics)</term><term>Protozoan Proteins (administration & dosage)</term><term>Protozoan Proteins (biosynthesis)</term><term>Protozoan Proteins (genetics)</term><term>Protozoan Proteins (immunology)</term><term>Rhizobium (genetics)</term><term>Tobacco (genetics)</term><term>Tobacco (immunology)</term><term>Tobacco (metabolism)</term><term>Toxoplasma (genetics)</term><term>Toxoplasma (immunology)</term><term>Toxoplasmosis (immunology)</term><term>Toxoplasmosis (prevention & control)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Anticorps antiprotozoaires (immunologie)</term><term>Antigènes de protozoaire (administration et posologie)</term><term>Antigènes de protozoaire (biosynthèse)</term><term>Antigènes de protozoaire (génétique)</term><term>Antigènes de protozoaire (immunologie)</term><term>Encéphale (anatomopathologie)</term><term>Encéphale (immunologie)</term><term>Encéphale (parasitologie)</term><term>Expression des gènes (MeSH)</term><term>Femelle (MeSH)</term><term>Feuilles de plante (génétique)</term><term>Feuilles de plante (immunologie)</term><term>Feuilles de plante (métabolisme)</term><term>Immunoglobuline G (immunologie)</term><term>Kystes (anatomopathologie)</term><term>Kystes (immunologie)</term><term>Kystes (parasitologie)</term><term>Potexvirus (génétique)</term><term>Protéines de protozoaire (administration et posologie)</term><term>Protéines de protozoaire (biosynthèse)</term><term>Protéines de protozoaire (génétique)</term><term>Protéines de protozoaire (immunologie)</term><term>Rhizobium (génétique)</term><term>Souris (MeSH)</term><term>Souris de lignée C3H (MeSH)</term><term>Tabac (génétique)</term><term>Tabac (immunologie)</term><term>Tabac (métabolisme)</term><term>Toxoplasma (génétique)</term><term>Toxoplasma (immunologie)</term><term>Toxoplasmose (immunologie)</term><term>Toxoplasmose (prévention et contrôle)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Antigens, Protozoan</term><term>Protozoan Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antigens, Protozoan</term><term>Protozoan Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Protozoan</term><term>Protozoan Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Protozoan</term><term>Antigens, Protozoan</term><term>Immunoglobulin G</term><term>Protozoan Proteins</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Antigènes de protozoaire</term><term>Protéines de protozoaire</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Encéphale</term><term>Kystes</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Antigènes de protozoaire</term><term>Protéines de protozoaire</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Leaves</term><term>Potexvirus</term><term>Rhizobium</term><term>Tobacco</term><term>Toxoplasma</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes de protozoaire</term><term>Feuilles de plante</term><term>Potexvirus</term><term>Protéines de protozoaire</term><term>Rhizobium</term><term>Tabac</term><term>Toxoplasma</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiprotozoaires</term><term>Antigènes de protozoaire</term><term>Encéphale</term><term>Feuilles de plante</term><term>Immunoglobuline G</term><term>Kystes</term><term>Protéines de protozoaire</term><term>Tabac</term><term>Toxoplasma</term><term>Toxoplasmose</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Brain</term><term>Cysts</term><term>Plant Leaves</term><term>Tobacco</term><term>Toxoplasma</term><term>Toxoplasmosis</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plant Leaves</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Feuilles de plante</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Encéphale</term><term>Kystes</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Brain</term><term>Cysts</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Brain</term><term>Cysts</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Toxoplasmosis</term></keywords><keywords scheme="MESH" qualifier="prévention et contrôle" xml:lang="fr"><term>Toxoplasmose</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Gene Expression</term><term>Mice</term><term>Mice, Inbred C3H</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Expression des gènes</term><term>Femelle</term><term>Souris</term><term>Souris de lignée C3H</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We adapted a previously described Agrobacterium-mediated transient expression system to test the expression level of three constructs carrying the surface antigen 1 (SAG1) of Toxoplasma gondii. Two constructs were based in a Potato virus X (PVX) amplicon. In one of them, the PVX movement protein genes were replaced by the sag1 gene. In the other, the sag1 gene was placed under the control of an additional coat protein subgenomic promoter. In the third construct, the sag1 gene was fused to an apoplastic peptide signal under the CaMV 35S promoter. Western blot analysis of leaf extracts infiltrated with each construct revealed a protein of 35 kDa. SAG1 accumulation in leaves ranged from 0.1 to 0.06% of total soluble protein (equivalent to 10 microg and 6 microg of SAG1 per gram of fresh leaf tissue, respectively). Three of five human seropositive samples reacted with tobacco-expressed SAG1 in Western blot analysis. The C3H mice were immunized with SAG-expressing leaf extracts and perorally challenged with a nonlethal dose of the T. gondii Me49 strain. Mice vaccinated with SAG1 showed significantly lower brain cyst burdens compared to those from the control group. Immunization with SAG1-expressing leaves elicited a specific humoral response with predominant participation of type IgG2a. In conclusion, a functional SAG1 version could be transiently expressed in tobacco leaves.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15805575</PMID><DateCompleted><Year>2006</Year><Month>05</Month><Day>10</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1073-6085</ISSN><JournalIssue CitedMedium="Print"><Volume>30</Volume><Issue>1</Issue><PubDate><Year>2005</Year><Month>May</Month></PubDate></JournalIssue><Title>Molecular biotechnology</Title><ISOAbbreviation>Mol Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Production of the main surface antigen of Toxoplasma gondii in tobacco leaves and analysis of its antigenicity and immunogenicity.</ArticleTitle><Pagination><MedlinePgn>41-50</MedlinePgn></Pagination><Abstract><AbstractText>We adapted a previously described Agrobacterium-mediated transient expression system to test the expression level of three constructs carrying the surface antigen 1 (SAG1) of Toxoplasma gondii. Two constructs were based in a Potato virus X (PVX) amplicon. In one of them, the PVX movement protein genes were replaced by the sag1 gene. In the other, the sag1 gene was placed under the control of an additional coat protein subgenomic promoter. In the third construct, the sag1 gene was fused to an apoplastic peptide signal under the CaMV 35S promoter. Western blot analysis of leaf extracts infiltrated with each construct revealed a protein of 35 kDa. SAG1 accumulation in leaves ranged from 0.1 to 0.06% of total soluble protein (equivalent to 10 microg and 6 microg of SAG1 per gram of fresh leaf tissue, respectively). Three of five human seropositive samples reacted with tobacco-expressed SAG1 in Western blot analysis. The C3H mice were immunized with SAG-expressing leaf extracts and perorally challenged with a nonlethal dose of the T. gondii Me49 strain. Mice vaccinated with SAG1 showed significantly lower brain cyst burdens compared to those from the control group. Immunization with SAG1-expressing leaves elicited a specific humoral response with predominant participation of type IgG2a. In conclusion, a functional SAG1 version could be transiently expressed in tobacco leaves.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Clemente</LastName><ForeName>Marina</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>IIB-INTECH, Camino de Circunvalación Laguna Km. 6, B7130IIWA, Chascomús, Prov. Buenos Aires, Argentina. mclemente@intech.gov.ar</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Curilovic</LastName><ForeName>Roberto</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Sassone</LastName><ForeName>Alina</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Zelada</LastName><ForeName>Alicia</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Angel</LastName><ForeName>Sergio O</ForeName><Initials>SO</Initials></Author><Author ValidYN="Y"><LastName>Mentaberry</LastName><ForeName>Alejandro N</ForeName><Initials>AN</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Biotechnol</MedlineTA><NlmUniqueID>9423533</NlmUniqueID><ISSNLinking>1073-6085</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000913">Antibodies, Protozoan</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000953">Antigens, Protozoan</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007074">Immunoglobulin G</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015800">Protozoan Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C047168">SAG1 antigen, Toxoplasma</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000913" MajorTopicYN="N">Antibodies, Protozoan</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000953" MajorTopicYN="N">Antigens, Protozoan</DescriptorName><QualifierName UI="Q000008" MajorTopicYN="N">administration & dosage</QualifierName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001921" MajorTopicYN="N">Brain</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003560" MajorTopicYN="N">Cysts</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007074" MajorTopicYN="N">Immunoglobulin G</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008809" MajorTopicYN="N">Mice, Inbred C3H</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="Y">Plant Leaves</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017863" MajorTopicYN="N">Potexvirus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015800" MajorTopicYN="N">Protozoan Proteins</DescriptorName><QualifierName UI="Q000008" MajorTopicYN="N">administration & dosage</QualifierName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012231" MajorTopicYN="N">Rhizobium</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="Y">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" 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PubStatus="entrez"><Year>2005</Year><Month>4</Month><Day>5</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15805575</ArticleId><ArticleId IdType="pii">MB:30:1:041</ArticleId><ArticleId IdType="doi">10.1385/MB:30:1:041</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Trends Plant Sci. 2001 May;6(5):219-26</Citation><ArticleIdList><ArticleId IdType="pubmed">11335175</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Res. 1990 Jul;16(3):293-305</Citation><ArticleIdList><ArticleId IdType="pubmed">2392880</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6286-90</Citation><ArticleIdList><ArticleId IdType="pubmed">8692807</ArticleId></ArticleIdList></Reference><Reference><Citation>Vaccine. 1998 Aug;16(13):1336-43</Citation><ArticleIdList><ArticleId IdType="pubmed">9682399</ArticleId></ArticleIdList></Reference><Reference><Citation>Protein Expr Purif. 2001 Oct;23(1):33-7</Citation><ArticleIdList><ArticleId IdType="pubmed">11570843</ArticleId></ArticleIdList></Reference><Reference><Citation>Vaccine. 2001 Mar 21;19(17-19):2749-55</Citation><ArticleIdList><ArticleId IdType="pubmed">11257419</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Appl Biochem. 2001 Feb;33(Pt 1):35-45</Citation><ArticleIdList><ArticleId IdType="pubmed">11171034</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1988 Nov 25;16(22):10929-30</Citation><ArticleIdList><ArticleId IdType="pubmed">3205733</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2002 Jun;20(6):622-5</Citation><ArticleIdList><ArticleId IdType="pubmed">12042869</ArticleId></ArticleIdList></Reference><Reference><Citation>Infect Immun. 1994 Jan;62(1):203-9</Citation><ArticleIdList><ArticleId IdType="pubmed">8262628</ArticleId></ArticleIdList></Reference><Reference><Citation>Vaccine. 2003 Jan 30;21(7-8):803-8</Citation><ArticleIdList><ArticleId IdType="pubmed">12531364</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Appl Biochem. 1999 Oct;30 ( Pt 2):113-6</Citation><ArticleIdList><ArticleId IdType="pubmed">10512789</ArticleId></ArticleIdList></Reference><Reference><Citation>Infect Immun. 2002 Feb;70(2):1014-6</Citation><ArticleIdList><ArticleId IdType="pubmed">11796643</ArticleId></ArticleIdList></Reference><Reference><Citation>Diagn Microbiol Infect Dis. 2003 Jan;45(1):45-52</Citation><ArticleIdList><ArticleId IdType="pubmed">12573550</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Diagn Lab Immunol. 1996 May;3(3):355-7</Citation><ArticleIdList><ArticleId IdType="pubmed">8705683</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Parasitol. 2003 May;33(5-6):479-93</Citation><ArticleIdList><ArticleId IdType="pubmed">12782049</ArticleId></ArticleIdList></Reference><Reference><Citation>Am J Trop Med Hyg. 1987 May;36(3):517-22</Citation><ArticleIdList><ArticleId IdType="pubmed">3578650</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11128-33</Citation><ArticleIdList><ArticleId IdType="pubmed">10500141</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 1992 Jul;2(4):549-57</Citation><ArticleIdList><ArticleId IdType="pubmed">1344890</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):11745-9</Citation><ArticleIdList><ArticleId IdType="pubmed">1465391</ArticleId></ArticleIdList></Reference><Reference><Citation>Diagn Microbiol Infect Dis. 2003 Dec;47(4):609-13</Citation><ArticleIdList><ArticleId IdType="pubmed">14711483</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1989 Apr 5;264(10):5569-74</Citation><ArticleIdList><ArticleId IdType="pubmed">2925621</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2000 Nov;18(11):1151-5</Citation><ArticleIdList><ArticleId IdType="pubmed">11062432</ArticleId></ArticleIdList></Reference><Reference><Citation>Infect Immun. 2003 Sep;71(9):5436-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12933900</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Parasitol. 2000 Nov;30(12-13):1217-58</Citation><ArticleIdList><ArticleId IdType="pubmed">11113252</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Drug Targets Immune Endocr Metabol Disord. 2002 Oct;2(3):227-34</Citation><ArticleIdList><ArticleId IdType="pubmed">12476487</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2000 Nov;18(11):1167-71</Citation><ArticleIdList><ArticleId IdType="pubmed">11062435</ArticleId></ArticleIdList></Reference><Reference><Citation>J Am Vet Med Assoc. 1990 Jan 15;196(2):259-62</Citation><ArticleIdList><ArticleId IdType="pubmed">2404925</ArticleId></ArticleIdList></Reference><Reference><Citation>Vaccine. 2003 Oct 1;21(27-30):4201-9</Citation><ArticleIdList><ArticleId IdType="pubmed">14505899</ArticleId></ArticleIdList></Reference><Reference><Citation>Cancer Res. 2002 Jul 1;62(13):3654-8</Citation><ArticleIdList><ArticleId IdType="pubmed">12097270</ArticleId></ArticleIdList></Reference><Reference><Citation>Infect Immun. 2000 Jan;68(1):38-45</Citation><ArticleIdList><ArticleId IdType="pubmed">10603366</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 1998 Oct;16(10):934-8</Citation><ArticleIdList><ArticleId IdType="pubmed">9788349</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Diagn Lab Immunol. 2004 Jul;11(4):704-10</Citation><ArticleIdList><ArticleId IdType="pubmed">15242945</ArticleId></ArticleIdList></Reference><Reference><Citation>Microbes Infect. 2003 Apr;5(5):457-62</Citation><ArticleIdList><ArticleId IdType="pubmed">12738002</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Parasitol. 2001 Dec;31(14):1699-703</Citation><ArticleIdList><ArticleId IdType="pubmed">11730799</ArticleId></ArticleIdList></Reference><Reference><Citation>Vaccine. 2000 May 22;18(23):2512-21</Citation><ArticleIdList><ArticleId IdType="pubmed">10775785</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 1998 Nov 16;17(22):6739-46</Citation><ArticleIdList><ArticleId IdType="pubmed">9822616</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biotechnol. 1998 Dec 11;66(2-3):137-46</Citation><ArticleIdList><ArticleId IdType="pubmed">9866865</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Argentine</li></country></list><tree><noCountry><name sortKey="Angel, Sergio O" sort="Angel, Sergio O" uniqKey="Angel S" first="Sergio O" last="Angel">Sergio O. Angel</name><name sortKey="Curilovic, Roberto" sort="Curilovic, Roberto" uniqKey="Curilovic R" first="Roberto" last="Curilovic">Roberto Curilovic</name><name sortKey="Mentaberry, Alejandro N" sort="Mentaberry, Alejandro N" uniqKey="Mentaberry A" first="Alejandro N" last="Mentaberry">Alejandro N. Mentaberry</name><name sortKey="Sassone, Alina" sort="Sassone, Alina" uniqKey="Sassone A" first="Alina" last="Sassone">Alina Sassone</name><name sortKey="Zelada, Alicia" sort="Zelada, Alicia" uniqKey="Zelada A" first="Alicia" last="Zelada">Alicia Zelada</name></noCountry><country name="Argentine"><noRegion><name sortKey="Clemente, Marina" sort="Clemente, Marina" uniqKey="Clemente M" first="Marina" last="Clemente">Marina Clemente</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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